Fig. 7: Proteomics analyses of autoantigens.
From: Antigen presentation by B cells enables epitope spreading across an MHC barrier
A Schematic overview of experimental approach. B Volcano plot of mean differences in log2[iBAQ values] of clone 564 C11 pulldowns (n = 4) and murine serum IgG pulldowns (n = 4). The line at -log10(q value) = 2 indicates the significance threshold for paired t-test at p = 0.01. C Plot of PC1 and PC2 for principal component analysis of five bridge chimeras, five no bridge chimeras, a pool of five 564Igi homozygous sera, and a pool of six C57BL/6J sera. D Heatmap of individual hits for the samples represented in the PCA analysis in C. Some graphical elements of A were created with BioRender.
