Fig. 4: O-acetylated (O-Ac) N-glycans derived from equine nasal tissue. | Nature Communications

Fig. 4: O-acetylated (O-Ac) N-glycans derived from equine nasal tissue.

From: Sialic acid O-acetylation patterns and glycosidic linkage type determination by ion mobility-mass spectrometry

Fig. 4: O-acetylated (O-Ac) N-glycans derived from equine nasal tissue.

a Extracted ion chromatograms (EIC) of B3 ions of PNGaseF released glycans from one of the equine nasal tissue, analyzed by HPLC-IM-MS (n = 3). b ATDs of single charged B1 and B3 fragment ions of di- (I) and mono-acetylated N-glycans (I) released from equine nasal tissue (n = 1). The O-acetylated N-glycan structures were assigned using accurate mass values; Sialic acid linkage and acetyl ester positions were determined by the CCS values of B1 and B3 ions.

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