Fig. 6: RTFL18 antagonizes the stabilization of PIF4 under low R:FR conditions. | Nature Communications

Fig. 6: RTFL18 antagonizes the stabilization of PIF4 under low R:FR conditions.

From: Shade-induced RTFL/DVL peptides negatively regulate the shade response by directly interacting with BSKs in Arabidopsis

Fig. 6

a The PIF4 protein levels in Col-0, dvl1-1D, and rtfl18*rtfl21 lines grown under white light (WL) and low R:FR conditions. b, c The PIF4 protein levels in the Col-0, bsk3, BSK3ox #4, bsk6, and BSK6ox #6 lines under WL and low R:FR conditions. d, e The PIF4 protein levels in the Col-0, dvl1-1D, dvl1-1D*BSK3ox #8, and dvl1-1D*BSK6ox #26 lines under WL and low R:FR conditions. f Pictures of the Col-0, dvl1-1D, PIF4ox, and dvl1-1D*PIF4ox lines under WL and low R:FR conditions. g The petiole lengths of the cotyledons in the Col-0, dvl1-1D, PIF4ox, and dvl1-1D*PIF4ox lines grown under WL and low R:FR conditions. h The petiole lengths of the first and second leaves in the Col-0, dvl1-1D, PIF4ox, and dvl1-1D*PIF4ox lines grown under WL and low R:FR. In a–e, the right panels show the quantification analysis of three biological replicates of PIF4 protein levels after normalization to β-tubulin/ Rbcl. Data are presented as mean values +/− SD (n = 3, n refers to biological replicates). The PIF4 protein levels of Col-0 under low R:FR were set as 1. Whole seedlings were used for experiments. Western blots were probed with an anti-PIF4 antibody. The levels of β-tubulin/ Rbcl indicate the loading concentration. In g and h, the numbers in the bar charts represent n of each sample. Data are presented as mean values +/− SEM. Letters indicate significant differences between mean values (two-way ANOVA: Tukey’s multiple comparisons test, P < 0.01), and groups with the same letters are not significantly different. Scale bars represent 5 mm. Source data are provided as a Source Data file.

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