Fig. 1: Scheme for generating thioether-based methacrylate covalent peptide or protein binders.
From: A simple method for developing lysine targeted covalent protein reagents
A cysteine residue can be introduced into peptides (pink ribbon) or recombinant proteins (light green), which are then modified using ethyl 2-(bromomethyl)acrylate. The resulting electrophile reacts with lysine or cysteine side chains proximal to the binding site on the receptor (light blue), to yield covalent adducts.
