Fig. 2: bAC CARTs mediate highly efficacious mRNA delivery to Jurkat cells.
From: Charge-altering releasable transporters enhance mRNA delivery in vitro and exhibit in vivo tropism
a Schematic representation of bAC monomer synthesis. b Polymerization scheme of bAC CARTs with TU/DBU catalyst system (see Methods section). c Jurkat transfection with eGFP mRNA (200 ng mRNA/100,000 cells) comparing MTC-1a, ONA, and bAC-1a CARTs. (n = 3, bars represent mean values +/− SD). Statistical significance was calculated using two-way ANOVA. d Chemical structures of bAC CART library. e Chemical structures of MTC CART analogs. d, e The numbers in red indicate lipidic block length, represented by the subindex “m”, and numbers in blue indicate cationic block length, and represented by the subindex “n”. f Particle size of bAC CART/mRNA complexes (n = 3, bars represent mean values +/− SD) and g Particle size comparison to MTC CART/mRNA complexes (n = 3, bars represent mean values +/− SD). h eGFP expression of bAC CARTs in Jurkat cells (100 ng mRNA/100,000 cells). Dotted line indicates ONA eGFP expression level as baseline, and red arrows indicate the top three performers (n = 4, bars represent mean values +/− SD). i Area under the curve (AUC) computation of eGFP expression (left) and eGFP+ AUC (right) levels among the best CARTs (n = 4, bars represent mean values +/− SD). Statistical significance was calculated using one-way ANOVA with Tukey’s multiple comparisons test.
