Fig. 5: bAC CARTs induce faster change in nanoparticle surface charge and earlier protein expression. | Nature Communications

Fig. 5: bAC CARTs induce faster change in nanoparticle surface charge and earlier protein expression.

From: Charge-altering releasable transporters enhance mRNA delivery in vitro and exhibit in vivo tropism

Fig. 5: bAC CARTs induce faster change in nanoparticle surface charge and earlier protein expression.

a Structures of CARTs included in this study. b Kinetics of surface charge change upon CART/mRNA complexation (top, n = 3, bars represent mean values +/− SEM). The bar graph on the bottom represents the average rate of surface charge change from 0–30 min. (n = 3, bars represent mean values +/− SEM). Statistical significance was calculated using one-way ANOVA with Tukey’s multiple comparisons test. c Schematic representation of methods to monitor mRNA release from CART/mRNA nanoparticles. Created with ChemDraw. d Kinetics of mRNA release upon CART/mRNA complexation (n = 3, bars represent mean values +/− SEM). e Kinetics of Cy5+ labeled mRNA uptake (top) and eGFP mRNA protein expression (bottom) in primary T cells with CART delivery (n = 4, bars represent mean values +/− SEM).

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