Fig. 2: SidH binding to tRNA is sequence independent.
From: Structural basis for the toxicity of Legionella pneumophila effector SidH
a The pie chart shows different type of tRNAs came in bound state to purified SidH from E. coli. tRNA isotypes with their percentage of abundance and color scheme (right panel). Source data are provided as a Source Data file. b The electrostatic potential surface projection of SidH shows that T-loop of tRNA (pink) inserts into positively charged groove (blue) of SidH. c The cartoon representation of the complex shows that the binding groove for tRNA is composed of distinct helical bundles colored and labeled as explained in the main text. d The detailed view of tRNA-binding region of SidH shows that the side chains of positively charged residues are pointed towards negatively charged phosphodiester backbone of tRNA to make ionic interactions.
