Fig. 2: Addition of various stress remodelers provides an increase in multiple ncAA incorporation.

a Schematic representation of iRFP-GFP^39TAG,149TAG and iRFP-GFP^{39TAG,149TAG,182TAG} reporters. GCE realization enables installation of two or three ncAAs into the corresponding reporter protein. Legend is the same as in Fig. 1a, c. b Heat maps illustrating the effect of adding various stress remodelers on efficiency of multiple ncAA incorporation in units of relative efficiency^GFP (%). Percentage relative efficiency^GFP is calculated the same way as in Fig. 1d. Median GFP signals were obtained after FC analysis of corresponding samples. The heat maps show the mean value for the relative efficiencies of three independent experiments. Source data are provided as a Source Data file. c FC analysis of the iRFP-GFP^39TAG,149TAG and iRFP-GFP^{39TAG,149TAG,182TAG} reporter in the absence and presence of the tested stress remodelers. Concatenated data from three independent experiments (300 ng tRNA^Pyl plasmid) are shown.