Fig. 4: IL-11 Mutein is an effective inhibitor of human IL-11 signalling in vitro.

A Representative dose-response curve for IL-11 or IL-11 variant stimulation. The EC₅₀ for IL-11_Δ10 was 0.010 ± 0.002 nM; the EC₅₀ for IL-11_Δ10/W147A was 0.6 ± 0.2 nM, the EC₅₀ for IL-11_Δ10/PAIDY was 0.14 ± 0.02 nM; the EC₅₀ for IL-11_Δ10/Mutein could not be determined. Data are presented as the mean ± SEM of three technical replicates. Representative of n = 3 independent experiments. Replicate experiments are presented in Supplementary Fig. 9A. B Representative dose-response curve for the inhibition of IL-11_Δ10 stimulation by the IL-11 variants IL-11_Δ10/W147A, IL-11_Δ10/PAIDY, and IL-11_Δ10/Mutein. The IC₅₀ for IL-11_Δ10/Mutein inhibition was 850 ± 275 nM, the IC₅₀ for the remaining variants was not determined. Data are presented as the mean ± SEM of three technical replicates. Representative of n = 3 independent experiments. Replicate experiments are presented in Supplementary Fig. 9B. C Potent inhibition of IL-11_Δ10 signalling by IL-11_Δ10/Mutein in the indicated human cancer cell lines. Data are presented as the mean ± SEM of three technical replicates. Representative of n = 2 independent experiments. Replicate experiments are presented in Supplementary Fig. 9C. Source data are provided as a Source Data file.