Fig. 2: APCMin/RasV12-transformed cells develop into diffusive carcinomas and invade lymphatic vessels. | Nature Communications

Fig. 2: APCMin/RasV12-transformed cells develop into diffusive carcinomas and invade lymphatic vessels.

From: Wnt activation disturbs cell competition and causes diffuse invasion of transformed cells through NF-κB-MMP21 pathway

Fig. 2: APCMin/RasV12-transformed cells develop into diffusive carcinomas and invade lymphatic vessels.The alternative text for this image may have been generated using AI.

a, b Confocal microscopic images of intestinal villi from APCMin-Villin-GFP, Villin-RasV12, or APCMin-Villin-RasV12 mice. APCMin-Villin-GFP, Villin-RasV12, or APCMin-Villin-RasV12 mice were injected with 1 mg tamoxifen and were sacrificed 21 days (a) or 36 days (b) later. Frozen sections were stained with DAPI (blue), anti-GFP antibody (green), and E-cadherin (red, b). c HE and immunostaining of intestinal villi from APCMin-Villin-RasV12 mice bearing carcinomas 42 days after RasV12 expression. Paraffin-embedded sections were processed for HE staining or were stained with GFP or E-cadherin antibody (brown). d 3D image of whole mount-stained intestinal villi from APCMin-Villin-RasV12 mice bearing carcinomas. A fixed sample was stained with Hoechst 33342 (blue), GFP (green), LYVE1 (red), and MECA32 (white). White arrows depict APCMin/RasV12 cells that invade lymph vessels. e HE and immunostaining of mesenteric lymph nodes of APCMin-Villin-RasV12 mice. Paraffin-embedded sections were processed for HE staining or were stained with DAPI (blue), GFP (green), and E-cadherin antibody (red). Scale bars, 50 μm (a, c, e) and 100 μm (b, d).

Back to article page