Fig. 2: Inhibition of TARP-γ8-selective AMPARs abolishes the acute effects of ketamine in the ventral hippocampus. | Nature Communications

Fig. 2: Inhibition of TARP-γ8-selective AMPARs abolishes the acute effects of ketamine in the ventral hippocampus.

From: Enhanced TARP-γ8-PSD-95 coupling in excitatory neurons contributes to the rapid antidepressant-like action of ketamine in male mice

Fig. 2

a Structures of the mechanism of JNJ55511118. b Preference for sucrose in the SPT (n = 11, 10, and 12 in Vehicle, 1 μM and 10 μM, respectively).c Immobility time in the FST (n = 15, 15, and 19 in Vehicle, 1 μM, and 10 μM, respectively). d Total distance in the OFT (n = 12, 13, and 14 in Vehicle, 1 μM, and 10 μM, respectively). e, f Schematic diagram of experiment (e) and histological verification of the cannula placement in the ventral hippocampus with Nissl staining (f). Scale bar = 500 μm. g Social interaction ratio of SIT (n = 9, 9, 9, 9, 10, 10, 10, and 8 in CON-ACSF-Vehicle, CON-ACSF-Ketamine, CON-JNJ55511118-Vehicle, CON-JNJ55511118-Ketamine, CSDS-ACSF-Vehicle, CSDS-ACSF-Ketamine, CSDS-JNJ55511118-Vehicle and CSDS-JNJ55511118-Ketamine, respectively). h Preference for sucrose in the SPT (n = 10 mice per group). i Immobility time in the FST (n = 10, 10, 10, 10, 8, 8, 10 and 10 in CON-ACSF-Vehicle, CON-ACSF-Ketamine, CON-JNJ55511118-Vehicle, CON-JNJ55511118-Ketamine, CSDS-ACSF-Vehicle, CSDS-ACSF-Ketamine, CSDS-JNJ55511118-Vehicle and CSDS-JNJ55511118-Ketamine, respectively). j Total distance traveled in the OFT (n = 10, 10, 10, 10, 10, 10, 10 and 9 in CON-ACSF-Vehicle, CON-ACSF-Ketamine, CON-JNJ55511118-Vehicle, CON-JNJ55511118-Ketamine, CSDS-ACSF-Vehicle, CSDS-ACSF-Ketamine, CSDS-JNJ55511118-Vehicle and CSDS-JNJ55511118-Ketamine, respectively). k Representative traces of AMPARs-mediated mEPSC recordings in ventral hippocampal CA1 neurons. l, m Quantification of cumulative probability and amplitude (l) and frequency (m) of mEPSCs (n = 8 cells from 4 mice in ACSF, n = 8 cells from 4 mice in Ketamine, n = 11 cells from 5 mice in JNJ55511118, n = 8 cells from four mice in JNJ55511118-Ketamine). Comparisons were performed by one-way ANOVA analysis followed by Dunnett’s multiple comparisons test in b–d, by two-way and one-way ANOVA analysis followed by Bonferroni’s multiple comparisons test in (g–j) and in (l, m), respectively. Data are presented as mean ± SEM. All numbers (n) are biologically independent experiments. Source data are provided as a Source Data file.

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