Fig. 5: CaMKIIα-dependent TARP-γ8-PSD-95 coupling is necessary for the rapid antidepressant effects of ketamine. | Nature Communications

Fig. 5: CaMKIIα-dependent TARP-γ8-PSD-95 coupling is necessary for the rapid antidepressant effects of ketamine.

From: Enhanced TARP-γ8-PSD-95 coupling in excitatory neurons contributes to the rapid antidepressant-like action of ketamine in male mice

Fig. 5: CaMKIIα-dependent TARP-γ8-PSD-95 coupling is necessary for the rapid antidepressant effects of ketamine.The alternative text for this image may have been generated using AI.

a The representative image of western blot. b The quantification of protein expression of p-CaMKIIα and CaMKIIα (n = 9 per group). c Preference for sucrose in the SPT (n = 9, 8, 9, and 10 in Vehicle-ACSF, Vehicle-KN93, Ketamine-ACSF, and Ketamine-KN93, respectively). d, e Immobility time in the FST (d) and total distance in the OFT (e) (n = 9, 9, 10, and 8 in Vehicle-ACSF, Vehicle-KN93, Ketamine-ACSF and Ketamine-KN93, respectively). f Representative western blot image of Co-IP assay. g Quantification of association between PSD-95 and TARP-γ8 (n = 8, 7, 7, and 9 in Vehicle-ACSF, Vehicle-KN93, Ketamine-ACSF and Ketamine-KN93, respectively). h, i Total protein expression of PSD-95 (h) and TARP-γ8 (i) (n = 8 samples per group). j Representative traces of AMPARs-mediated mEPSC recordings in ventral hippocampal CA1 neurons from different groups. k, l Quantification of cumulative probability and amplitude (k) and frequency (l) of mEPSCs (n = 8 cells from 4 mice in Vehicle-ACSF, n = 7 cells from four mice in Vehicle-KN93, n = 7 cells from four mice in Ketamine-ACSF and n = 8 cells from 4 mice in Ketamine-KN93). m Representative western blot image of Co-IP assay. n Quantification of the association between PSD-95 and TARP-γ8 (n = 7 in Tat-Scrambled, n = 8 in Tat-TTPV). o Preference for sucrose in the SPT (n = 9, 9, 8 and 8 in Vehicle-Tat-Scrambled, Vehicle-Tat-TTPV, Ketamine-Tat-Scrambled and Ketamine-Tat-TTPV, respectively). p, q Immobility time in the FST (p) and total distance in the OFT (q) (n = 10, 9, 8 and 8 in Vehicle-Tat-Scrambled, Vehicle-Tat-TTPV, Ketamine-Tat-Scrambled and Ketamine-Tat-TTPV, respectively). Comparisons were performed by unpaired, two-tailed t test in (b, n) and two-way ANOVA analysis followed by Bonferroni’s multiple comparisons test in (c–l, o–q). Data are presented as mean ± SEM. All numbers (n) are biologically independent experiments. Source data are provided as a Source Data file.

Back to article page