Fig. 7: CC-GEMS as a platform for therapeutic ligand production.
From: Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells
a Schematic representation of CC-GEMS platform utilized for IL-10 secretion. HEK293T cells are transiently transfected to express A-typeJAK/STAT receptors with α2, STAT3 and IL-10 under the control of STAT3 recognition element (see Supplementary Fig. S35 and “Methods”). IL-10 is secreted upon the addition of SUMO-tagged A’-A’ dipeptide with l2. b IL-10 quantification measured by ELISA (see “Methods”) in HEK293T cells transiently transfected with A-typeJAK/STAT receptors with linker α2 (8 aa, GS repeats), and afterwards incubated with 0.12 μM purified SUMO-tagged ligand A’-A’ or Γ’-Γ’ with linker l2 (see Supplementary Fig. S5b) or no ligand (“Methods”). IL-10 calibration curve is shown in Supplementary Fig. S36. Individual data points represent independent triplicates, performed on the same day. Fold change and significance (one-way ANOVA with Tukey’s multiple comparison test) is noted above bars. ns: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (see Supplementary Table S4). Source data are provided as a Source Data file.
