Fig. 4: Neuronal activity dysfunction in the RN glutamatergic neurons of control and RS rats.
From: Stress-induced red nucleus attenuation induces anxiety-like behavior and lymph node CCL5 secretion
a Schematic of the fiber photometry setup. Ca2+ transients were recorded from RNGCaMP6 rats. b Schematic of fiber implantation in GCaMP6-expressing RN glutamatergic neurons. Scale bar, 100 µm, n = 6. c GCaMP6 signals from RN glutamatergic neurons aligned to the moment of exploratory behavior of the central zone in the open field. Control: black; RS: red. Gray areas indicate rats in the central zone. d Difference in average fluorescence between control rats and RS-treated rats. The average fluorescence in RS-treated rats was significantly lower than that in control rats (two-tailed unpaired t test, t = 9.082, P < 0.0001, n = 6 rats per group). e, f Heatmap of the RN glutamatergic neuronal response to exploratory behavior in control and RS-treated rats. g, h Averaged calcium signals in response to exploratory behavior in control and RS-treated rats. Black and red lines, mean calcium signal during exploratory behavior; gray area and red area, SEM. i Quantification of the change in calcium signals from exploratory behavior in control and RS-treated rats (two-tailed unpaired t test, t = 3.180, P = 0.0038, n = 6 rats per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file.
