Fig. 7: Through β1-involvement, progesterone similarly dilates cerebral arteries of male and female mice when evaluated in vivo.
From: Progesterone activation of β1-containing BK channels involves two binding sites
a Cranial window images of C57BL/6J male MCA before any infusion (pre-infusion) and after infusion of 10 μM PROG or 10 μM PROG-oxime via the carotid artery. b Cranial window images of C57BL/6J female MCA before any infusion (pre-infusion) and after infusion of 10 μM PROG or 10 μM PROG-oxime via the carotid artery. c Averaged fold-changes in male MCA diameter comparing the responses to 10 μM PROG and 10 μM PROG-oxime for each minute of recording compared to baseline diameter determined from the image taken immediately prior to any infusion. A horizontal dashed line from 1 (y-axis), underscores no change in artery diameter. A black arrow indicates the beginning of infusion. Number of data points (n)=5 for each group, each data point was recorded from a separate animal. Data are shown as mean ± SEM. *Statistically significant from vehicle control (P = 0.0158, 0.0079, 0.0079, 0.007, 0.0111, 0.0119, 0.011 and 0.007; two-sided Mann–Whitney U-test). d Averaged fold-changes in female MCA diameter comparing the responses to 10 μM PROG and 10 μM PROG-oxime for each minute of recording compared to baseline diameter determined from the image taken immediately prior to any infusion. A horizontal dashed line from 1 (y-axis), underscores no change in artery diameter. A black arrow indicates the beginning of infusion. Number of data points (n)=5 for each group, each data point was recorded from a separate animal. Data are shown as mean ± SEM. *Statistically significant from vehicle control (P = 0.0095, 0.0119, 0.007, 0.0079, 0.0079, 0.0063 and 0.0069; two-sided Mann–Whitney U-test). e Averaged percentage of maximal artery changes in vivo produced by PROG and PROG-oxime in male C57BL/6 mice. Data are shown as mean ± SEM. Geometric shapes indicate individual records. *Statistically significant from vehicle control (P = 0.012; two-sided Mann–Whitney U-test). f Averaged percentage of maximal artery changes in vivo produced by PROG and PROG-oxime in female C57BL/6 mice. Data are shown as mean ± SEM. Geometric shapes indicate individual records. *Statistically significant from vehicle control (P = 0.007; two-sided Mann–Whitney U-test).
