Fig. 1: ALT cells display elevated levels of telomeric dilncRNAs.

a Telomeric dilncRNAs were quantified by strand-specific RT-qPCR and normalized on RPLP0 gene; average values for each cell line were quantified in three biologically independent experiments; data are presented as mean values +/− SEM among n = 2 non-ALT and n = 3 ALT cell lines; two-tailed unpaired t-test, df = 3, t = 1.174 teloG, t = 3.718 teloC; *p = 0.0339. b Northern blot analysis of total RNA. Radiolabeled C-rich and G-rich telomeric oligonucleotides were used to probe for teloG and teloC dilncRNAs, respectively. EtBr-stained 18S RNA is shown as loading control; n = 2 biologically independent experiments, one representative image shown. c Telomeric dilncRNAs were quantified and reported as in (a); mean values for each cell line were quantified in n = 2 or 3 biologically independent experiments; data are presented as mean values +/− SEM among n = 5 non-ALT and ALT cell lines; two-tailed unpaired t-test, df = 8, t = 3.295 teloG, t = 4.127 teloC; *p = 0.0109, **p = 0.0033. d HeLa Long Telomere (LT) cells were transfected with siSCR (control) or siASF1a/b siRNAs twice per week, and telomeric dilncRNAs were quantified as in (a) 14 days after first siRNA transfection; data are presented as mean values +/− SEM, n = 3 biologically independent experiments; two-tailed unpaired t-test, df = 4, t = 4.208 teloG, t = 3.432 teloC; for teloG *p = 0.0136, for teloC *p = 0.0265. Source data are provided as a Source data file.