Fig. 2: The structure of the Get1/Get2 heterodimer is conserved between H. sapiens and C. thermophilum.
From: The GET insertase exhibits conformational plasticity and induces membrane thinning
A Model for ctGet2ΔN-Get1/Get3 in A835 amphipol superposed to the cryo-EM density. Membrane plane defined perpendicular to the Get3 symmetry axis with boundaries inferred from the location of the TMDs. B Structure of ctGet2ΔN-Get1/Get3. The schematic shows the topology of ctGet1 and ctGet2, with dashed lines representing features not present in our model. C Superimposition of ctGet2ΔN-Get1 TMDs with hsGet2ΔN-Get1 (hsGet1 TMDs 1-3 and hsGet2 TMD3; RMSD 2.69 Å over 79 Cα atoms). The membrane planes are defined as in Figures (A–D). D Model for hsGet2ΔN-Get1/Get3 in PMAL-C8 amphipol superposed to the cryo-EM density reprocessed from EMDB-1026624. Membrane plane defined perpendicular to the Get3 symmetry axis with boundaries inferred from the location of the TMDs. E Side-by-side comparison of the ER cap (black) from hsGet1 (residues 126-150) and ctGet1 (residues 128-153) after superimposition as shown in (C).
