Fig. 6: Luminex mDC culture supernatant profiling. | Nature Communications

Fig. 6: Luminex mDC culture supernatant profiling.

From: Immuno-metabolic dendritic cell vaccine signatures associate with overall survival in vaccinated melanoma patients

Fig. 6: Luminex mDC culture supernatant profiling.The alternative text for this image may have been generated using AI.

A The Human Checkpoint 14-plex and immune profiling 65-plex assay kit (Thermo-Fisher ProcartaPlex) were used to measure immune-modulatory molecules in mDC culture supernatants from 4 healthy donors and 27 melanoma patients. Row labels include HD and patient response indications and absence (No) or presence (Yes) of patient-derived (MA)-specific CD8, CD4, combined CD8 + CD4 IFN-γ T-cell responses. B Forest plot summarizing linear regression analysis for immune-modulatory chemokine/cytokine levels with clinical outcome associations. Intercept points, P-values and 95% confidence intervals are indicated (HD, n = 4, Melanoma, n = 27). C Bar plots represent Spearman correlation coefficient (R) and p-values of mDC culture supernatants from 27 melanoma patients correlating with SCENITH metabolic parameters Glucose Dependence, and Seahorse assay glycolytic capacity and maximal oxygen consumption rate. Source data are provided as a Source Data file.

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