Fig. 8: Evaluation on DNA-PAE@BAY-876-mediated immunotherapy in vivo. | Nature Communications

Fig. 8: Evaluation on DNA-PAE@BAY-876-mediated immunotherapy in vivo.

From: Inhibition of glycolysis-driven immunosuppression with a nano-assembly enhances response to immune checkpoint blockade therapy in triple negative breast cancer

Fig. 8

a, b Flow cytometry detection on the tumor infiltration of Foxp3+ Tregs and IL−10+ Tregs. c Flow cytometry evaluation on the tumor-infiltration of total immune cells (CD45+). d Flow cytometry detection on the tumor infiltration of IFN-γ+CD8+ T cells. e Statistical analysis of data shown in panels a–d by flow cytometry (n = 3). (1) NC, (2) PAE, (3) aptCTLA-4, (4) aptPD-L1, (5) BAY-876, (6) DNA-PAE, (7) DNA-PAE@BAY-876. f Immunofluorescence images on the tumor-infiltration of CD4+ and CD8+ T cells in different groups with five mice per group. g Immunofluorescence images of IL-10 in tumor tissues after different treatment with five mice per group. h Photographs of lung metastasis inhibition in 4T1 tumor-bearing mice after different treatments with five mice per group. Experiments in panels a–d and f–h were repeated three times independently with similar results. Data are presented as mean values ± SEM (n = 3 mice for panel e). Statistical analysis was carried out via two-way ANOVA method. Source data are provided as a Source Data file.

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