Fig. 1: Nde1 promotes dynein motility together with Lis1.

a In vitro reconstitution of dynein motility using dynein, dynactin, BicDR1, Lis1, and Nde1 on biotinylated microtubules immobilized to the glass surface. The red star represents an LD655 dye attached to BicDR1 for TIRF imaging. Dynein, dynactin, and tubulin were not labeled with a fluorescent dye. b Representative kymographs show the motility of wtDDR in the presence of 0, 10, and 1000 nM Nde1 and the absence of Lis1. c Normalized run frequency distribution of wtDDR under different Nde1 concentrations. The center line and whiskers represent the mean and s.d., respectively (n = 20 microtubules for each condition). P values are calculated from a two-tailed t test. d Representative kymographs show the motility of wtDDR in the presence of 0–250 nM Lis1 and 0–1000 nM Nde1. e The run frequency of wtDDR in different Lis1 and Nde1 concentrations (mean ± s.d.; n = 20 microtubules for each condition). Results were normalized to the 0 nM Lis1 and 0 nM Nde1 condition. f Representative kymographs show the motility of mtDDR complexes with or without Nde1 and Lis1. g Run frequencies of wtDDR and mtDDR with different Nde1 and Lis1 concentrations (mean ± s.d.; n = 10 microtubules for each condition). Source data are provided as a Source Data file.