Fig. 3: Characterization of the barley diketone metabolism hydrolase (HvDMH) producing the key intermediates on the β-diketone synthesis pathway. | Nature Communications

Fig. 3: Characterization of the barley diketone metabolism hydrolase (HvDMH) producing the key intermediates on the β-diketone synthesis pathway.

From: Biosynthesis of barley wax β-diketones: a type-III polyketide synthase condensing two fatty acyl units

Fig. 3: Characterization of the barley diketone metabolism hydrolase (HvDMH) producing the key intermediates on the β-diketone synthesis pathway.The alternative text for this image may have been generated using AI.

A Total ion chromatogram of lipids from E. coli expressing HvDMH, after transesterification into methyl esters (MEs) and conversion of hydroxyl groups into trimethylsilyl (TMS) ethers. Homolog series of saturated (sat.) and monounsaturated (unsat.) 2-ketones (orange), 2-alkanols (purple), 3-hydroxyacid MEs (red) and fatty acid MEs (gray) were detected. B Amounts of 2-ketones in E. coli expressing HvDMH (quantified against internal standard C21 2-ketone). C Amounts of 2-alkanols in E. coli expressing HvDMH (quantified against internal standard C21 2-alkanol). D Amounts of 3-hydroxyacid MEs in E. coli expressing HvDMH (quantified against internal standard C20 2-hydroxyacid ME and normalized to C16 3-hydroxyacid ME). E Amounts of fatty acid MEs in E. coli expressing HvDMH. Data are presented as means ± standard deviations of three biological replicates. Source data are provided as a Source Data file.

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