Fig. 7: Subcellular localization of HvDMH and HvDMP.

Confocal microscope images of barley protoplasts transiently expressing Pro35S:HvDMH-GFP, showing A HvDMH-GFP, B chloroplast autofluorescence, C bright field, and D the merged image of (A–C). Confocal microscope images of N. benthamiana leaves coexpressing, E Pro35S:GFP-HvDMP, and F the ER marker Pro:35S:HDEL-RFP, and G merged image of (E, F). Bars = 10 μm. Both experiments were independently repeated three times and 10–15 samples/regions were investigated each time with similar results.