Fig. 6: T. brucei infection leads to expansion of IL-17A+ cells in the iWAT during early infection. | Nature Communications

Fig. 6: T. brucei infection leads to expansion of IL-17A+ cells in the iWAT during early infection.

From: IL-17 signalling is critical for controlling subcutaneous adipose tissue dynamics and parasite burden during chronic murine Trypanosoma brucei infection

Fig. 6: T. brucei infection leads to expansion of IL-17A+ cells in the iWAT during early infection.The alt text for this image may have been generated using AI.

A Uniform manifold approximation and projection (UMAP) of 46,546 high-quality cells from a single-cell RNA sequencing (scRNAseq) dataset. B Frequency plot showing changes in cell frequency under naive or infectious conditions. C Dot plot representing the expression levels of top marker genes used to catalogue the diversity of cells within the dataset. The side of the dots represents the percentage of cells that express a given marker, and the colour intensity represents the level of expression. D Dot plot representing Il17a expression in cell clusters. E UMAP of subset T cell clusters. F Dot plot representing the expression levels of top marker genes used to categorise T cell populations. G Flow cytometry analysis of the proportion of all live IL-17A+ cells. Frequency of H TH17, and I TCRγδ+ T cells in the iWAT. Frequency of (J) CD27- and (K) CD27+ γδ T cells in the iWAT. Single-cell data are comprised of one technical replicate per condition, each containing pooled cells from the iWAT of five male mice replicate. For flow cytometry data, data, biological replicates are indicated by symbols for each panel, and data were analysed using a two-tailed Student’s t test. Data for all panels are expressed as mean ± SD.

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