Fig. 3: Determination of the CPD-mediated C-terminal azidation of Endo-F3(D165A) under different conditions (100 μM of Endo-F3(D165A)-CPD, 5 mM of 3-azidopropylamine, x μM of InsP6, buffers, 4 °C, 5 h). | Nature Communications

Fig. 3: Determination of the CPD-mediated C-terminal azidation of Endo-F3(D165A) under different conditions (100 μM of Endo-F3(D165A)-CPD, 5 mM of 3-azidopropylamine, x μM of InsP6, buffers, 4 °C, 5 h).

From: C-terminal modification and functionalization of proteins via a self-cleavage tag triggered by a small molecule

Fig. 3

a With different InsP6 concentration in PBS. b In PB with different pH. c Under different buffers (final pH = 8.0). P: product; H: hydrolyzed.

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