Fig. 4: Screening of the catalysts for accelerating the CPD-mediated C-terminal functionalization (100 μM of Endo-F3(D165A)-CPD, 2 mM of 3-azidopropylamine, 5 μM of InsP6, x mM of catalyst, in NaOAc buffer (final pH=8.0)). | Nature Communications

Fig. 4: Screening of the catalysts for accelerating the CPD-mediated C-terminal functionalization (100 μM of Endo-F3(D165A)-CPD, 2 mM of 3-azidopropylamine, 5 μM of InsP6, x mM of catalyst, in NaOAc buffer (final pH=8.0)).

From: C-terminal modification and functionalization of proteins via a self-cleavage tag triggered by a small molecule

Fig. 4

a MS spectra profiles of the CPD-mediated C-terminal azidation of Endo-F3(D165A) with different catalysts (5 mM of catalyst, 4 °C, 3 h). b Time course of protein modification with different concentrations of DMAP (0-10 mM, 4 °C, 3 h). c MS spectra profiles of the CPD-mediated C-terminal azidation of Endo-F3(D165A) with different temperatures (5 mM of DMAP, 4 °C or 25 °C, 5 h). P: product; H: hydrolyzed.

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