Fig. 1: Cohesin is enriched at human pericentromeres but not α-satellite HOR arrays.
From: Defining a core configuration for human centromeres during mitosis
a Linear binding profile of three cohesin subunits - RAD21, SA1, SA2 and CTCF, mapped to T2T CHM13 v1.0, across the length of chromosome 18 is displayed. Centromere haplotype (CenHap) of chromosome 18 denoted in red. Peaks called by macs are denoted as gray tracks. Data is an average representation of 3 biological replicates. b Zoomed in view of centromere 18 CenHap with 10 Mb flanking regions (Cen Adjacent) is shown. CENP-A ChIP peaks are enriched within the D18Z1 α-satellite HOR array (red). Cohesin subunits lack binding within the α-satellite HOR array but are enriched outside of the HOR array in the pericentromeric domains within the CenHap. c Peak densities (number of peaks per Mb) of CENP-A, SA1, SA2 and RAD21 within active α-satellite HOR array (asHOR), CenHap, 2 Mb regions flanking CenHap (CenAdj) and 100 random genomic regions outside of the centromere haplotype (nonCen) of chromosome 18 in CHM13 cells is shown. d Metagenome analysis of peak densities (log2) of CENP-A, SA1, SA2 and RAD21 within the above stated regions, across all chromosomes is shown. e Mean proportion of mapped reads for RAD21, SA1 and SA2 in nonCen, asHOR and CenHap regions of the CHM13 genome is shown. Data show mean of 3 biologically independent replicates. Error bars denote S.D. f Peak densities (log2) of CTCF in the above stated regions of the CHM13 genome mirroring peak densities of cohesin factors. For all box plots, upper and lower whiskers represent the largest and smallest values no further than 1.5 * inter quartile range (IQR) of the bounds, respectively. Data outside of whiskers are outlying points. Center denotes median, and lower and upper bounds of box denote 25th and 75th percentile of peak densities, respectively.
