Fig. 3: Single particle averaging of 3D-SIM images with aligned CENP-A and RAD21 signals.
From: Defining a core configuration for human centromeres during mitosis
a CENP-A paired clusters were identified with an automated algorithm and their centers were used to align all centromeres. Centroids of CENP-A signals were joined, and the center of this line was defined as the center of each centromere. 60px × 60px surrounding 463 individual centromeres (18 spreads) were cropped and signals from all images were summed. b Summed intensities of DAPI, CENP-A and RAD21 around centromeres are shown (n = 463 chromosomes). x and y axes indicate distance (nm). c–e Intensities (white overlay line in (b) of CENP-A measured along the lateral axis and RAD21 measured along the lateral and along the chromosomal axes are shown (black closed circles in (c–e)), along with their Gaussian fits (magenta line). Dotted lines represent positions of peaks from the fits. Arrow indicates the dip in RAD21 intensity at the center of the vertical axis.
