Fig. 2: Detection of S protein.
From: Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes
a Representative current-time traces for the translocation of SBA encoded 10 kbp DNA molecular probe (end-modified) in the absence (i) and presence (ii) of S protein (20 nM). Zoom-in views of typical translocation events are shown below current-time traces. b Representative current-time traces for concentration-dependent experiments with the addition of S protein of 0.2, 2, 20, 200 pM, 2, 20, and 200 nM, respectively. c Density scatter plots of dwell time versus peak current amplitude for the translocation of the molecular probe only (blue, n = 1023) and protein/DNA conjugates (orange, n = 123). d Binding curve of molecular probe incubated with S protein ranging from 0 to 200 nM. e Comparison of the detection of S protein (200 nM) from SARS-CoV-2 virus, HCoV-NL63 and HCoV-OC43. All translocation experiments were performed with 200 pM molecular probe in 2 M LiCl buffer (5 mM MgCl2, 10 mM Tris–HCl, 1 mM EDTA, pH = 8) at an applied potential bias of 300 mV. Error bars in d and e represent the standard deviation of three independent replicates and the measure of the centre represents their corresponding mean value. Source data are provided as a Source Data file.
