Fig. 3: Nanopore detection of N protein.
From: Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes
a Schematics of NBA modified 10 kbp DNA molecular probe and its binding to N protein. Representative translocation events are shown underneath. Upon binding, the N protein produces a secondary peak superimposed on the dsDNA level at a fractional position of ≈0.43 for a typical event. b Binding curve of molecular probe incubated with N protein ranging from 0 to 20 nM. c Comparison of the detection of N protein (20 nM) from SARS-CoV-2 virus and seasonal flu virus of HCoV-229E and HCoV-OC43. d, e Scatter plots of secondary peak amplitude versus dwell time (d) or fractional position (e) and corresponding statistics (n = 107). All the translocation experiments were performed with 200 pM molecular probe in 2 M LiCl, 5 mM MgCl2, 10 mM Tris–HCl, 1 mM EDTA, and pH 8 solution at an applied bias of 300 mV. Error bars in b and c represent the standard deviation of three independent experimental repeats and the measure of the centre represents their corresponding mean value. Source data are provided as a Source Data file.
