Fig. 3: HPA functions as an antidote.
From: A toxin-antidote system contributes to interspecific reproductive isolation in rice
a Target site in HPA (top) for CRISPR/Cas9-mediated mutagenesis and a HPA genomic fragment from Mer (bottom) for genetic complementation test. PAM is highlighted in red. b Pollen fertility in knock-out (hpa-CR) and complementation (gHPA-COM) lines. Data are means ± SD (n = 5 independent spikelets). **P = 1.21003E-10 of NIL-qHMS1 vs hpa-CR-1, 2.27263E-5 of F1 vs hpa-CR-2, 0.00075 of F1 vs gHPA-COMs < 0.01 by two-tailed student’s t test. c–h Representative images of pollen phenotype in wild type (WT) (c) and knock-out (d) of NIL-qHMS1, and knock-out (e), WT (f) and complemented (g, h) lines of F1 (DJY1/ NIL-qHMS1). Scale bar, 100 μm. i Observed segregation rate among progeny of the complemented lines, showing successful transmission of the DJY1 allele of qHMS1. **P = 9.613E-7 < 0.01 in χ² test. Source data are provided as a Source Data file.
