Fig. 3: Energy landscape of uncoating state transitions.
From: A tradeoff between enterovirus A71 particle stability and cell entry
a, b WT and K162E were incubated at different temperatures for 60 min. At the indicated time points, virus titers were determined by plaque assay (a) and proportion of viral RNA release determined by RNase-sensitivity assay (b). Model of the reaction pathway for the N-to-A and A-to-E transitions c Reaction pathway for thermally mediated conversion for WT and K162E. In this model, we assume that the pathway proceeds through a single transition state (T.S.) whose free energy of activation ΔG‡ is composed by entropy of activation ΔS‡ and enthalpy of activation ΔH‡, which were estimated using the Eyring model. The horizontal dashed lines represent the energy barrier for the uncatalyzed reaction. Size of the arrow indicates ΔS‡ and ΔH‡ calculated for each reaction, and values of each component of the reaction are given in kcal/mol.
