Fig. 8: Apoptotic death of skin and brain endothelial cells in embryos induced to express EPHB4 F867L alone within the vasculature during mid-to late gestation.
From: Mutation of key signaling regulators of cerebrovascular development in vein of Galen malformations
Embryos of indicated genotypes (n = 3) were administered tamoxifen at E13.5 and harvested at E17.5. Sections of skin (a) and brain (b) were stained with antibodies against CD31, active caspase-3, and collagen IV (Coll IV). Note active caspase-3 positive endothelial cells in blood vessels of skin and brain of Ephb4fl/F867L Cdh5-CreERT2 embryos (arrowheads) but not in littermate Ephb4fl/F867L controls. Note also intracellular accumulation of collagen IV in skin vascular endothelial cells of Ephb4fl/F867L Cdh5-CreERT2 embryos (arrows) but not in controls. c Mean +/-1 SEM of % of vessels with at least one apoptotic endothelial cell per field of the choroidal plexus/cerebellum region (Ephb4 fl/F867L skin, n = 5; Ephb4 fl/F867L Cdh5-ert2cre skin, n = 7; Ephb4 fl/F867L brain, n = 5; Ephb4 fl/F867L Cdh5-ert2cre brain, n = 3 independent fields). **p = 0.0025; *p = 0.0179; two-sided Mann–Whitney test.
