Fig. 2: Hormonal regulation of HSP47 by insulin and corticosteroids.

a Relative protein expression of HSP47 in 3T3-L1 adipocytes after insulin (100 nM; p < 0.0001) or dexamethasone (Dex;100 nM; p < 0.0052) treatments for 48 h (n = 4 each). b, Relative protein expression of HSP47 in ex vivo cultured mouse adipose tissue after insulin (100 nM; p < 0.0001) or dexamethasone (100 nM; p < 0.0001) treatments for 48 h (n = 4 each). c Relative protein expression of HSP47 in 3T3-L1 adipocytes after insulin (Ins; 100 nM; ctrl vs Insulin p = 0.0013) treatment with/without insulin receptor inhibitor (OSI-906; IRi; 0.2 µM; Insulin vs Ins.+ IRi, p = 0.0019) for 48 h (n = 3 each). d Relative protein expression of HSP47 in 3T3-L1 adipocytes after dexamethasone (100 nM; p = 0.0003) treatments with/without glucocorticoid receptor antagonist (RU486; GRi; 10 µM; Dex vs Dex+GRi, p = 0.0005) for 48 h (n = 3 each). e Relative protein expression of HSP47 in mouse adipose tissue after insulin receptor inhibitor (OSI-906; IRi; 100 mg/kg) treatment for 24 h (n = 3 each; p = 0.0045). f Relative protein expression of HSP47 in mouse adipose tissue of control (flox/flox) or adipose-specific GR knockout (AGRKO) mice after 2 weeks of corticosterone treatment (n = 8; p < 0.001). g Relative gene expression of Hsp47 in chicken adipose tissue after short-term 5 h fasting (Fast.) or ad libitum feeding (Feed.) with/without insulin neutralization by porcine anti-insulin serum (GSE35581; n = 5 each; Fast. vs Feed. p = 0.0194; Feed. vs anti-Insulin, p = 0.0215). h Relative gene expression of HSP47 in ex vivo cultured human omental adipose tissue with dexamethasone (0, 1, 10, 1000 nM; n = 3; 10 nM, p = 0.0014; 1000 nM, p = 0.0002) or cortisol (200 nM; n = 1) (GSE88966). Data represent the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001; n refers to sample size. Statistical significance was determined by Tukey–Kramer test (a–d, g, h) and two-tailed unpaired t-test (e and f). Source data are provided as a Source Data file.