Fig. 2: The FBF-2 CT competes with partner proteins for binding to the RBD. | Nature Communications

Fig. 2: The FBF-2 CT competes with partner proteins for binding to the RBD.

From: Intra- and inter-molecular regulation by intrinsically-disordered regions governs PUF protein RNA binding

Fig. 2

Representative isothermal titration calorimetry thermograms (top, differential power [DP] vs time) and corresponding titration curve-fitting graphs (bottom) are shown for interaction between a FBF-2 RBD and FBF-2 CT, c FBF-2 RBD + CT and LST-1 A, d FBF-2 RBD + CT and LST-1 B, e FBF-2 RBD and FBF-2 CT L610A, f FBF-2 RBD + CT L610A and LST-1 A, and g FBF-2 RBD + CT L610A and LST-1 B. Data points on the curve-fitting graphs are shown as open circles. b Previously reported mean Kd from two independent replicates with similar results for interaction between FBF-2 RBD and LST-1 A (top) and FBF-2 RBD and LST-1 B (bottom)18. Mean Kd from two independent replicates with similar results are indicated. Thermodynamic parameters are summarized in Supplementary Table 3. Source data are provided as a Source Data file.

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