Fig. 4: Electrostatic repulsion by acidic residues in the FBF-2 CT reduces RNA-binding affinity. | Nature Communications

Fig. 4: Electrostatic repulsion by acidic residues in the FBF-2 CT reduces RNA-binding affinity.

From: Intra- and inter-molecular regulation by intrinsically-disordered regions governs PUF protein RNA binding

Fig. 4

a Surface representation of an AlphaFold model of FBF-2 RBD + CT colored by electrostatic potential, and the CT PIM is colored yellow. A cartoon of cFBE RNA was added by superimposing the AlphaFold model with the crystal structure of FBF-2 RBD + CT. The path of RNA 5′ to the cFBE is indicated by a dashed line. b FBF-2 CT sequence with acidic residues (red) and PIM (yellow). c Analysis of conformational fluctuations in FBF-2 RBD + CT. Root mean square fluctuations (RMSF) are plotted by FBF-2 RBD + CT residues for apo and RNA bound states. d Representative EMSA gel and binding curve are shown for binding of FBF-2 RBD + CT Loop6A mutant to FBEa RNA. The left lane is RNA only. Data points on the graph are shown as filled circles. Mean Kd from three independent technical replicates with similar results is indicated. See also Supplementary Table 4. Source data are provided as a Source Data file.

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