Fig. 1: Uterine epithelial-specific deletion of Shp2 leads to female dystocia with abnormal hormone levels and PGF2α synthesis.

a Coimmunostaining of SHP2 and CK8 in Shp2^f/f and Shp2^d/d mouse uteri on day 19. LE luminal epithelum, MU muscle. b Pregnant length in Shp2^f/f (n = 5) and Shp2^d/d (n = 8) female mice. Data represent as mean ± SEM. Two-tailed unpaired student’s t-test, **p = 0.0014. Each plot represents an individual sample. c Survival rates of pregnant Shp2^f/f and Shp2^d/d mother. The numbers in columns indicate survival pregnant females over total number of pregnant females. ***p = 8.46e−04, Fisher’s exact test. d Serum estradiol-17β (E2) level in Shp2^f/f (n = 13) and Shp2^d/d (n = 13) mice on day 19. Data represent as mean ± SEM. Two-tailed unpaired student’s t-test, ***p = 5.41e−05. e Serum progesterone (P4) level in Shp2^f/f (n = 16) and Shp2^d/d (n = 17) mice on day 19. Data represent as mean ± SEM. Two-tailed unpaired student’s t-test, ***p = 5.15e−06. f In situ hybridization of Akr1c18 in Shp2^f/f and Shp2^d/d ovaries on day 19. This result was repeated independently in three individual mice with similar results. g Western blot of STAR levels in Shp2^f/f and Shp2^d/d ovaries on day 19. β-ACTIN was used as a loading control, n = 4. h Immunofluorescence of COX2 in decidua and epithelium of Shp2^f/f and Shp2^d/d mice on day 19. Mat maternal tissue, LE luminal epithelum. i PGF2α level in Shp2^f/f (n = 14) and Shp2^d/d (n = 11) mouse uteri on day 19. Data represent the mean ± SEM. Two-tailed unpaired student’s t-test, ***p = 1.89e-07. Each plot represents an individual sample. j Sm-FISH of Cox1 in Shp2^f/f and Shp2^d/d mice on day 19. This result was repeated independently in three individual mice with similar results. k Western blot of COX1 levels in Shp2^f/f and Shp2^d/d ovaries on day 19. β-ACTIN was used as a loading control, n = 4. l Sm-FISH of Akr1b3 in Shp2^f/f and Shp2^d/d mice on day 19 with immunofluorescence of E-cadherin. m Pregnant length after injection of PBS (n = 12) and PGF2α (n = 12, 50 μg/mouse) intraperitoneally in Shp2^d/d mice on day 19. Data are shown as boxplots where midlines indicate medians, boxes indicate interquartile range and whiskers indicate minimum/maximum range. n The ratio of survival pregnant females after injection of PBS (n = 10) and PGF2α (n = 12, 50 μg/mouse) intraperitoneally in Shp2^d/d mice on day 19. Source data of b, c, d, e, g, i, k and n are provided as a Source Data file.