Fig. 5: Haspin knockout does not influence the displacement of endogenous TAF3 from chromosomes in mitosis.

A Immunofluorescence microscopy (with formaldehyde fixation) for DNA (blue), TAF3 (green), CENP-C (centromeres, red), and H3T3ph (gray) in wild type and Haspin knockout HeLa cells. The experiment was performed once, but the result was confirmed using GFP-TAF3 and Haspin inhibitor treatment (see Supplementary Fig. 6B). Scale bars = 10 µm. B Quantification of results in A. The ratio of TAF3 and H3T3ph staining intensity to DNA staining intensity in chromatin-containing regions, relative to that in the cytosol, was determined. TAF3 intensity was normalized to that in wild type interphase nuclei; and H3T3ph intensity was normalized to that on wild type mitotic chromatin. Bars represent mean ± SD. Statistical analysis was carried out using non-normalized data by unpaired two-tailed Student t tests. ***p = 3.2 × 10⁻⁸; ns not significant (p = 0.99); n = 20 interphase cells and 10 mitotic cells. Source data including exact p values are provided as a Source Data file.