Fig. 7: Haspin knockout does not influence the displacement of endogenous ING2, Dido3, or LSD1 from chromosomes in mitosis.

A Immunofluorescence microscopy (with methanol fixation) for DNA (blue), ING2 (green), CENP-C (centromeres, red), and H3T3ph (gray) in wild type and Haspin knockout HeLa cells. Immunofluorescence staining was carried out independently twice with methanol fixation, and 3 times with formaldehyde fixation (see Supplementary Fig. 9A). B As for A, but staining for Dido3 (green). Immunofluorescence staining was carried out once. C As for A, but staining for LSD1 (green). Immunofluorescence staining was carried out once with methanol fixation, and three times independently with formaldehyde fixation (see Supplementary Fig. 9B). Scale bars = 10 µm.