Fig. 1: Activity of Simkania USP enzymes. | Nature Communications

Fig. 1: Activity of Simkania USP enzymes.

From: Functional and structural diversity in deubiquitinases of the Chlamydia-like bacterium Simkania negevensis

Fig. 1

a Domain scheme of SnUSP1-3. Gray box indicates the DUB domain, bracket indicates the purified construct. b–d Activity-based probe reaction of SnUSP1 b, SnUSP2 c, or SnUSP3 d with Ub-PA, ISG15CTD-PA, SUMO1-PA, SUMO3-PA and NEDD8-PA. Asterisks (*) mark the shifted band after reaction. e AMC activity assay with SnUSP1-3 at the indicated concentrations against Ub-AMC substrate shown as released fluorescence (RFU) over time (min). The shown RFU values are the means of triplicates. f–j Ubiquitin chain cleavage analysis. A panel of di-ubiquitin chains was treated with 0.5 µM SnUSP1 f, 0.025 µM SnUSP1 g, 0.5 µM SnUSP2 h, 0.1 µM SnUSP2 i, or 5 µM SnUSP3 j for the indicated time points. Source data are provided as a Source Data file.

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