Fig. 2: Activity of Simkania Josephin-type enzymes. | Nature Communications

Fig. 2: Activity of Simkania Josephin-type enzymes.

From: Functional and structural diversity in deubiquitinases of the Chlamydia-like bacterium Simkania negevensis

Fig. 2

a Domain scheme of SnJos1-2. Gray box indicates the DUB domain, bracket indicates the purified construct. b, c Activity-based probe reaction of SnJos1 b or SnJos2 c with Ub-PA, ISG15CTD-PA, SUMO1-PA, SUMO3-PA, and NEDD8-PA. d AMC activity assay with SnJos1-2 against the Ub-AMC substrate shown as released fluorescence (RFU) over time (min). The shown RFU values are the means of triplicates. e–f Ubiquitin chain cleavage analysis. A panel of diubiquitin chains was treated with 5 µM SnJos1 d, or 5 µM SnJos2 e for the indicated time points. Source data are provided as a Source Data file.

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