Fig. 6: Activities of Simkania CE1. | Nature Communications

Fig. 6: Activities of Simkania CE1.

From: Functional and structural diversity in deubiquitinases of the Chlamydia-like bacterium Simkania negevensis

Fig. 6

a Domain scheme of SnCE1-5. The gray box indicates the DUB domain and the bracket indicates the purified construct. b Activity-based probe reaction of SnCE1 with Ub-PA, ISG15CTD-PA, SUMO1-PA, SUMO3-PA, and NEDD8-PA. c AMC activity assay with SnCE1 against Ub-/SUMO1-/SUMO2-AMC substrates is shown as released fluorescence (RFU) over time (min). The shown RFU values are the means of triplicates. d Ubiquitin chain cleavage analysis. A panel of diubiquitin chains was treated with 5 µM SnCE1 at the indicated time points. e Activity assay with SnCE1 against SUMO chains. A mixture of different SUMO chain lengths was treated with the DUB candidate at the indicated time points. Source data are provided as a Source Data file.

Back to article page