Fig. 6: TDRD3 prevents the accumulation of TOP3B DNA and RNA cleavage complexes. | Nature Communications

Fig. 6: TDRD3 prevents the accumulation of TOP3B DNA and RNA cleavage complexes.

From: The TDRD3-USP9X complex and MIB1 regulate TOP3B homeostasis and prevent deleterious TOP3B cleavage complexes

Fig. 6

a Scheme of the in vitro TOP3Bcc formation assay in steady-state condition. DNA or RNA oligo substrate labeled at the 3′-end with a fluorophore is incubated with recombinant TOP3B in the presence or absence of recombinant TDRD3. TOP3Bcc formation results in a slower migrating band and can be detected via the fluorophore at the 3′-ends of oligo construct4. b Reduced steady-state levels of DNA and RNA TOP3Bccs with increasing TDRD3 (stoichiometric ratio indicated below the gel). c Scheme of the TOP3Bcc reversal kinetics assay. TOP3Bccs formed (as indicated above) in the absence and presence of TDRD3 (TOP3B: TDRD3 = 1: 4) were diluted 3-fold and allowed to reverse for 0–30 min. d Time-course showing the stimulation of DNA and RNA TOP3Bcc reversal by TDRD3. Source data are provided as a Source Data file.

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