Fig. 3: n-BANKs promoted endothelial tube formation. | Nature Communications

Fig. 3: n-BANKs promoted endothelial tube formation.

From: Enhanced pericyte-endothelial interactions through NO-boosted extracellular vesicles drive revascularization in a mouse model of ischemic injury

Fig. 3: n-BANKs promoted endothelial tube formation.The alternative text for this image may have been generated using AI.

A Representative photomicrographs from a scratch-cell motility assay of SVEC4–10 cells in the present of 500 μM CoCl2 for the indicated times (n = 8). The scratch area reflected the endothelial cell migration ability. Scale bar, 400 μm. B Quantification of SVEC4–10 cell migration rate in a scratch wound assay (n = 8; ****P < 0.0001). C Tube formation was monitored periodically with images depicting endothelial tube formation 4 h after seeding (n = 15). The number and length of tubules assembled by elongation and joining of endothelial cells reflected the tubulogenesis ability of n-BANKs. Scale bar, 1000 μm. D Quantification of number of loops at 4 h (n = 15; Model, ***P = 0.0004; GTN, ***P = 0.0002). E–G Quantification of vascular progression (E vascular length; F number of branching points; G number of master junctions) at 4 h (n = 15; ****P < 0.0001). H Schematic illustration of endothelial tube formation activated by n-BANKs. Data are mean ± SD, *** is P < 0.001, **** is P < 0.0001 by one-way ANOVA test.

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