Fig. 6: n-BANKs recruited pericytes for enhancing the maturity of the newly formed blood vessels in CLI mouse model. | Nature Communications

Fig. 6: n-BANKs recruited pericytes for enhancing the maturity of the newly formed blood vessels in CLI mouse model.

From: Enhanced pericyte-endothelial interactions through NO-boosted extracellular vesicles drive revascularization in a mouse model of ischemic injury

Fig. 6: n-BANKs recruited pericytes for enhancing the maturity of the newly formed blood vessels in CLI mouse model.The alternative text for this image may have been generated using AI.

A Representative images and quantification showing NG2 expression and pericyte coverage in ischemic muscle sections (n = 3; NG2 intensity: Model *P = 0.0144, GTN*P = 0.0194; Pericyte density: Model **P = 0.0375, GTN *P = 0.0439). Scale bar, 100 μm. B qRT-PCR analysis of endothelial markers (CD31, CD34 and CD105) of muscle tissue form ischemic limbs at day 3 after treatment (n = 3; CD31, ***P = 0.0001; CD34, **P = 0.0021; CD105, **P = 0.0037). C Representative immunofluorescence images and quantification of ischemic gastrocnemius muscle sections stained with eNOS (green) and α-SMA (red) at day 3 after treatment (n = 17; Relative blood vessel diameter: Model versus GTN *P = 0.0212, Model versus n-BANK ****P < 0.0001; Fluorescence intensity of eNOS: Model versus GTN ***P = 0.0001, Model versus n-BANK ****P < 0.0001). Scale bar, 50 μm. D Western blot images showing total and phosphorylated protein levels of eNOS in muscle from ischemic hindlimb (n = 3). E NO level in ischemic hindlimb treated with n-BANKs (n = 3). Data are mean ± SD, * is P < 0.05, ** is P < 0.01, *** is P < 0.001, **** is P < 0.0001 by one-way or two-way ANOVA test.

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