Fig. 1: The effects of S. iranensis on barley seedlings under abiotic stress and the bioactive components produced by S. iranensis.
From: Streptomyces alleviate abiotic stress in plant by producing pteridic acids
a The box plots depict the plant height, fresh weight and dry weight of barley seedlings growing under osmotic stress mediated by 20% (w/v) PEG-6000 (mean ± SD, n = 18). b the box plots depict the plant height, fresh weight and dry weight of barley seedlings growing under salinity stress mediated by 100 mM NaCl (mean ± SD, n = 18). In a and b, statistical significance was assessed by one-way ANOVA with post hoc Dunnett’s multiple comparisons test. Asterisks indicate the level of statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Abbreviation: Mock: control; Si: treatment of S. iranensis culture broth; D: treatment of S. iranensis/ΔptaA culture broth; M: treatment of blank medium (ISP2). c The box plots depict the plant height, fresh weight and dry weight of barley seedlings growing under drought stress (mean ± SD, n = 18). Different colours of box plots indicate different growing conditions: green, 21 days water; brown, 7 days treatment after 7 days water followed by 7 days drought; red, 7 days water followed by 14 days drought. Statistical significance was assessed by one-way ANOVA with Tukey test. Asterisks indicate the level of statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Abbreviation: Water: well water for 21 days; CK, 7 days treatment of water after 7 days water + 7 days drought; Si: 7 days treatment of S. iranensis culture broth after 7 days water + 7 days drought; D: 7 days treatment of S. iranensis/ΔptaA culture broth after 7 days water + 7 days drought; M: treatment of blank medium (ISP2); Drought: 14 days drought after 7 days water. Statistical significance was assessed by one-way ANOVA with Tukey test. d The metabolite profile of the native S. iranensis growing in liquid ISP2 medium, the known secondary metabolites were identified by HR-LC-MS and highlighted; e the bioactive components pteridic acid H (1) and pteridic acid F (2) isolated from S. iranensis. All box plots with centre lines showing the medians, boxes indicating the interquartile range, and whiskers indicating a range of minimum to maximum data beyond the box. Source data are provided as a Source Data file.
