Fig. 6: Construction of an APS-mediated sulfotransferase activity indicating system.
From: Engineering sulfonate group donor regeneration systems to boost biosynthesis of sulfated compounds
a The reaction equation catalyzed by PPDK. b Schematic diagram of the APS-mediated sulfotransferase activity indicating system. c Absorbance detection and LC-MS detection were used to verify the APS-mediated sulfotransferases activity indicating system by using Stf0 as an example. APS adenosine-5′-phosphosulfate, PPDK pyruvate phosphate dikinase, LDHA lactate dehydrogenase A, PEP phosphoenolpyruvate, AMP adenosine 5′-monophosphate, PPi pyrophosphate, ATP adenosine 5′-triphosphate, NADH (NAD+), reduced (oxidized) nicotinamide adenine dinucleotide. All the data are expressed as the mean ± S.D. from three (n = 3) biologically independent replicates. Source data are provided as a Source Data file.
