Fig. 2: In vivo two-photon calcium imaging of retinal ganglion cell axon terminals in the mouse superior colliculus. | Nature Communications

Fig. 2: In vivo two-photon calcium imaging of retinal ganglion cell axon terminals in the mouse superior colliculus.

From: Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus

Fig. 2

a Schematic diagram of the experimental setup for retinal ganglion cell (RGC) axonal imaging in the mouse superior colliculus (SC). b Representative image of cranial window (n = 15 for axon imaging and 20 for somatic imaging). Medial-posterior part of SC was clearly visible through a cylindrical silicone plug attached to a glass coverslip. c Average intensity projection of representative axonal imaging data, overlaid with detected RGC axonal patches (n = 21; color-coded). See also Supplementary Movie 1. d Footprint of three representative RGC axonal patches (#18, 2, and 20 in distinct color; from left to right, respectively) overlaid with the profile of the rest patches (in gray). e Corresponding receptive field of the three representative RGC axonal patches (from d), estimated by reverse-correlation analysis (ellipse, 1 standard deviation Gaussian profile).

Back to article page