Fig. 5: Mice with selective fibroblast EGFR deletion developed less fibrosis after UUO. | Nature Communications

Fig. 5: Mice with selective fibroblast EGFR deletion developed less fibrosis after UUO.

From: Epidermal growth factor receptor activation is essential for kidney fibrosis development

Fig. 5: Mice with selective fibroblast EGFR deletion developed less fibrosis after UUO.The alternative text for this image may have been generated using AI.

a Immunoblotting indicated relatively lower total kidney EGFR levels in FibEGFR−/− mice at day 7 after UUO. n = 4 and 5. FibEGFR−/− mice exhibited less kidney fibrosis after UUO for 7 days as indicated by qPCR for kidney Acta2, Col1a1, Col4a1 and Fn (n = 6) (b), immunoblotting for PDGFRβ, Collagen IV (Col IV), and α-SMA (n = 4 and 5) (c), qPCR for Col1a1 and Col4a1 in isolated myofibroblasts (n = 6) (d), quantitative α-SMA immunostaining (n = 6) (e) and Picrosirius red staining (n = 5) (f). Data are means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, analyzed using two tailed Student’s t test for all.

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