Fig. 3: Pharynx-to-body length proportions are robust to tissue-specific depletion of RAGA-1.
From: Maintenance of appropriate size scaling of the C. elegans pharynx by YAP-1
a Experimental approach to deplete RAGA-1 in selected tissues. An AID tag was inserted into the endogenous locus of the raga-1 gene and Tir1 was expressed ubiquitously or under tissue-specific promoters. Addition of auxin to the growth medium leads to ubiquitination and proteasomal degradation of RAGA-1 in tissues expressing Tir1. b Schematic representation of tissue-specific promoters used to express Tir1. c Body (top) and pharynx (bottom) length as a function of time after depletion of RAGA-1 in indicated tissues. Color indicates IAA concentration from red to blue: no Tir1 expression 0 mM IAA, Tir1 expression + 0 mM, 0.1 mM, 0.25 mM, 0.5 mM, 1 mM IAA. d Scatter plot showing body vs. pharynx length at the beginning of L1 and at all larval moults M1 to M4 (circles in order from left to right) under pharyngeal (left) or epidermal (right) AID of RAGA-1. black circles: relation between pharynx and body length when unperturbed (no Tir1 expression and no IAA). Solid black line: linear regression to unperturbed body-to-pharynx length (P-line). Dashed black line: 5% deviation from P-line. Coloured circles: IAA concentrations from red to blue: 0 mM, 0.1 mM, 0.25 mM, 0.5 mM, 1 mM. Error bars are standard error of the mean among day-to-day repeats. Where error bars are invisible, they are smaller than the marker. Slope m of P-line from 0 to 1 mM IAA for pharyngeal RAGA-1 AID: 0.43, 0.41, 0.43, 0.43, 0.37; for epidermal RAGA-1 AID: 0.43, 0.45, 0.45, 0.45, 0.43; without Tir1 expression: 0.44, 0.43, 0.43, 0.43, 0.42. e Deviation of pharynx-to-body length ratio (deviation from P-line) vs. body length at 30% of L1 stage and at larval moults (mean of day-to-day repeats). Colours indicate tissues of Tir1 expression; orange: pharynx, purple: epidermis, green: no Tir1 expression. Marker symbol indicates IAA concentration: diamond: 0 mM, square: 0.1 mM, triangle: 0.25 mM, circle: 0.5 mM, asterisk: 1 mM. Error bars are standard errors among day-to-day repeats, where invisible, errors are smaller than the marker. c–e Number individuals n, 26 < n < 259 from number of day-to-day repeats m between 2 and 10. See Supplementary Tables 1 and 2 for n and m of each condition.
