Fig. 5: yap-1 is required for robustness of pharynx-to-body length proportions towards epidermal RAGA-1 inhibition.
From: Maintenance of appropriate size scaling of the C. elegans pharynx by YAP-1
a Pharynx vs. body length at 30% of L1 stage and at moults M1 to M4 under epidermal RAGA-1 AID (left) and without Tir1 expression (right) and with indicated RNAi. Black: mock control, red: yap-1, blue: dbl-1, lon-1, wts-1, ftt-2. Black line: linear regression to M1 to M4 of mock RNAi. Error bars in x and y: SEM among individuals. If no error bars are visible, they are smaller than the marker. b As (a), but for wild type and yap-1(tm1416) on OP50-1. Error bars are SD among individuals. c As (a), but for deviation of pharynx length from unperturbed conditions (deviation from P-line of the respective mock RNAi). p < 10−10 for comparison yap-1(RNAi) vs. mock RNAi for col-10p:tir1 strain at M1 to M4 (ranksum test, two-sided). d As (c), but for wild type and yap-1(tm1416) on OP50-1. Error bars: SEM among individuals. e Box plot of pharynx length at M4. First 4 boxes from left: yap-1(RNAi) with controls; right: yap-1(tm1416) with controls. central line: median, box: interquartile ranges (IQR), whisker: ranges except extreme outliers (>1.5*IQR), circles: individual data points. p < 10−8 for interaction between RNAi or mutant and RAGA-1 AID (2-way ANOVA). f As (e), but for body length at M4. g As (e), but for deviation of pharynx length from P-line of corresponding negative control. h Representative images (within 1% of the median pharynx length) of indicated genotypes and treatments. Scale bar = 0.1 mm. i As (e), but for duration of L4 stage. j As (e), but for mean body growth rate during L4. k Speculative model for the role of yap-1 in organ growth coordination: Attachment of the epidermis (brown) and the gastrointestinal tract (blue for intestine, green for pharynx) to the cuticle (black) causes forces (red) acting on cells of wrongly proportioned organs. As a mechanotransducer, YAP-1 may respond to these forces and induce an appropriate counteracting cellular growth response. a–g, i, j number of individuals are in Supplementary Table 2. Precise p-values and p-values for pairwise comparisons are in Supplementary Table 3.
